Plum pox potyvirus RNA replication in a crude membrane fraction from infected Nicotiana clevelandii leaves
نویسندگان
چکیده
منابع مشابه
The motif V of plum pox potyvirus CI RNA helicase is involved in NTP hydrolysis and is essential for virus RNA replication.
The plum pox potyvirus (PPV) protein CI is an RNA helicase whose function in the viral life cycle is still unknown. The CI protein contains seven conserved sequence motifs typical of RNA helicases of the superfamily SF2. We have introduced several individual point mutations into the region coding for motif V of the PPV CI protein and expressed these proteins in Escherichia coli as maltose bindi...
متن کاملA highly sensitive immunocapture polymerase chain reaction method for plum pox potyvirus detection.
A highly sensitive assay, based on polymerase chain reaction amplification of cDNA synthesized from the viral RNA of antibody-captured viral particles, has been developed for plum pox potyvirus (PPV) detection. The reaction, called immunocapture/PCR (IC/PCR), yields a specific 243-bp product. The immunocapture step, by allowing the use of large sample volumes and by the viral particle prepurifi...
متن کاملTotal RNA extraction method and Prunus species influence the detection of Plum pox potyvirus by real-time RT-PCR
Successful total RNA (totRNA) extraction is a prerequisite for a successful real-time PCR. In the present work we compared one manual and one automated totRNA extraction method for detection of Plum pox potyvirus (PPV) in leaves of different Prunus sp. using developed real-time RT-PCR assay. Advantages and disadvantages of compared methods are described in the view of sensitivity, reproducibili...
متن کاملNucleotide sequence of the 3'-terminal region of the RNA of the El Amar strain of plum pox potyvirus.
The nucleotide sequence of the 3'-terminal 4773 nucleotides of the RNA of a widely divergent, aphid-transmissible strain of plum pox potyvirus isolated from Egypt (PPV-El Amar) was determined. The sequenced region covers the carboxy terminus of the cylindrical inclusion (CI) gene, and the putative 6K protein, the NIa protease, the NIb RNA polymerase and the coat protein genes, linked together a...
متن کاملNucleotide Sequence of the Capsid Protein Gene of Plum Pox Potyvirus
The sequence of 1232 nucleotides from the 3' region of the plum pox virus (PPV) RNA has been determined. This sequence contains one long open reading frame (ORF). The sequence of the 17 amino-terminal amino acids of the PPV capsid protein was determined chemically. An identical amino acid sequence was found in the translation product of the large ORF, starting at amino acid 330, which is alanin...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1991
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-72-4-785